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Prokaryotic operons typically include a(n) ________ and a(n) ________ with multiple genes.


A) operator; terminator
B) operator; promoter
C) promoter; repressor
D) inducer; repressor
E) CAP-binding site; inducer

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DNA damage caused by nitrous acid results in ________ mutations.


A) insertion
B) substitution
C) deletion
D) frameshift
E) both insertion and deletion

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A protein has altered function as a result of a single amino acid substitution in the polypeptide.This change resulted from a (missense/nonsense/silent)mutation.

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A polypeptide in a wild type microbe contains the sequence Leu-Pro-Tyr-Ser-Pro.A phenotypic variant of the species has the peptide sequence Leu-Pro-Cys-Ser-Pro.This is an example of a(n) ________ mutation.


A) nonsense
B) missense
C) silent
D) frameshift
E) inversion

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Which of the following is characteristic of prokaryotic genomes but not eukaryotic genomes?


A) histones
B) circular chromosomes
C) linear chromosomes
D) enclosed in a nuclear membrane
E) typically consist of a few to several chromosomes

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The Ames test demonstrates that a chemical is


A) carcinogenic.
B) carcinogenic in Salmonella.
C) mutagenic in Salmonella.
D) carcinogenic in humans.
E) mutagenic in humans.

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Which of the following is involved in translation?


A) rRNA only
B) tRNA only
C) mRNA only
D) both mRNA and tRNA
E) mRNA, rRNA, and tRNA are all involved.

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Except during initiation of translation,transfer RNA molecules carrying amino acids initially bind to the ribosome at the (P/A/E)site.

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During elongation,a charged tRNA first enters the ribosomal ________ site and then moves into the ________ site.


A) A; E
B) P; A
C) P; E
D) A; P
E) E; A

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Most bacteria have a natural ability to take up DNA from their environment.

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The process of ________ requires participation of tRNA molecules.


A) DNA replication
B) capping
C) transcription
D) translation
E) gene regulation

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Prokaryotic cells are diploid.

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RNA polymerase is primarily responsible for


A) DNA replication.
B) translation.
C) transcription.
D) transformation.
E) polyadenylation.

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Which of the following is NOT a characteristic of both DNA and RNA polymerases?


A) directionality of synthesis
B) energy is provided by pyrophosphate
C) hydrogen bonding of complementary nucleotides
D) requirement for an initiation signal
E) requirement for a primer

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You observe a microbiologist examining two plates and notice the pattern of colonies are nearly identical with the exception of a few colonies that are absent on one of them.The plates likely were produced by


A) positive selection culturing.
B) replica plating.
C) pour plating.
D) streak plating.
E) the Ames test.

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