Question 1

Will a cloned calico cat have the exactly the same pattern of orange and black patches on her coat compare with her calico donor? Explain your answer.

Accepted Answer

No, a cloned calico cat will not have ex...

Question 2

Describe an experiment to determine whether the tomato you just bought from the grocery store is genetically modified. Show sample positive results.

Accepted Answer

(e.g. design primers specific ...

Question 3

You are trying to make a transgenic mouse but there is very poor survivorship of the embryos after introduction of the foreign gene by pronuclear injection. Describe a method you could use to get around this problem.

Accepted Answer

One method to improve survivorship of em...

Question 4

Discuss some of the pros and cons of using genetically modified nonhuman primates in experimental medicine.

Accepted Answer

Genetically modified nonhuman primates h...

Question 5

You have created a strain of knockout mice. Describe an experiment to confirm that the mice lack expression of the targeted gene at the level of mRNA expression. Show sample positive results.

Accepted Answer

To confirm that the knockout mice lack e...

Question 6

Which of the following is not a suspected reason for the inefficiency/poor outcomes of reproductive cloning?

Accepted Answer

A)   inefficient reprogramming
B)   premature terminal differentiation in the developing embryo
C)   telomere length
D)   chromosome missegregation during early embryonic divisionsE) A) and D)
F) A) and C)

Question 7

Mating mice from different founder lineages that carry identical transgenes will result in

Accepted Answer

A)   one transgene being expressed and the other silenced.
B)   a heterozygote for the transgene.
C)   a true homozygote in which independent segregation of the transgenic loci is predictable.
D)   a homozygote in which segregation of the transgenic loci is not necessarily independent.E) A) and B)
F) A) and C)

Question 8

All of the following are true of stem cells except:

Accepted Answer

A)   They can be found in many developing organs.
B)   They are terminally differentiated.
C)   They have a high replicative capacity.
D)   They may differentiate to replace cells lost to injury.E) A) and B)
F) B) and C)

Question 9

Describe the method for creating a transgenic mouse by pronuclear injection. What information can a transgenic mouse provide?

Accepted Answer

The method for creating a transgenic mou...

Question 10

The results of nuclear transplantation experiments in Xenopus laevis by John Gurdon and co-workers established the basic principle that

Accepted Answer

A)   cell differentiation depends on changes in the expression not content of the genome.
B)   cell differentiation depends on changes in the content not expression of the genome.
C)   cell differentiation depends on changes in both the expression and content of the genome.
D)   cloning by nuclear transfer is only possible in amphibians.E) A) and B)
F) A) and C)

Question 11

When making a mouse by gene targeting

Accepted Answer

A)   there is random integration of the transgene into the embryonic genome.
B)   there is disruption or mutation of a targeted gene in the embryonic genome.
C)   the resulting mice are genetically identical to the donor nucleus.
D)   the integration event into the embryonic genome usually occurs by nonhomologousrecombination.E) A) and B)
F) B) and D)

Question 12

Eduardo Kac the transgenic artist produced which of the following?

Accepted Answer

A)   Mickey the GFP mouse
B)   A sculpture of the first transgenic rat-sized mouse
C)   Alba the GFP bunny
D)   A photo montage of pronuclear microinjectionE) A) and B)
F) A) and D)

Question 13

Less than 1% of all nuclear transfers from adult differentiated cells result in normal-appearing cloned offspring. Give possible explanations for this lack of efficiency.

Accepted Answer

There are several possible explanations ...

Question 14

What information did the study of crown gall disease provide that was critical for plant genetic engineering?

Accepted Answer

A)   Understanding the role of plant hormones in growth promotion.
B)   Knowledge of plant tumor-inducing genes.
C)   Understanding the role of opine synthesis enzymes in tumor progression.
D)   Discovery of a plasmid that introduces virulence genes into plants.E) C) and D)
F) All of the above

Question 15

Gene knockouts often result in embryonic lethality. What strategy do investigators often use to make it possible to study a gene's role later in development?

Accepted Answer

A)   Gene knockout using an inducible tetracycline resistance gene.
B)   Use of embryonic stem cells from a strain of mice with brown fur and a blastocyst from astrain of mice with black fur.
C)   Implantation of embryos into a pseudopregnant female.
D)   Inducible Cre/Lox system for site-specific recombination.E) A) and D)
F) A) and C)

Question 16

Describe the method for creating a knockout mouse by gene targeting. Explain the importance of selectable marker genes in this procedure. What information can a knockout mouse provide?

Accepted Answer

Creating a knockout mouse by gene target...

Question 17

Which is not a method by which one can introduce foreign DNA into a plant cell?

Accepted Answer

A)   calcium chloride treatment followed by heat shock
B)   infection with a specific bacterium
C)   microballistic "gene gun"
D)   electroporationE) B) and D)
F) All of the above

Question 18

When cloning an organism by nuclear transfer

Accepted Answer

A)   there is random integration of a transgene into the recipient cell's nuclear genome.
B)   there is disruption or mutation of a targeted gene in the resulting organism.
C)   the resulting organism is genetically identical to only the donor nucleus and not the recipient cell's nucleus.
D)   the cytoplasm of the donor cell is transferred to the recipient cell.E) None of the above
F) B) and C)

Exam 15: Genetically Modified Organisms: Use in Basic and Applied Research

Which is not a method by which one can introduce foreign DNA into a plant cell?

Correct Answer
verified

All of the following are true of stem cells except:

Correct Answer
verified

Which of the following is not a suspected reason for the inefficiency/poor outcomes of reproductive cloning?

Correct Answer
verified

Describe the method for creating a transgenic mouse by pronuclear injection. What information can a transgenic mouse provide?

Correct Answer
Answered by ExamLex AI
The method for creating a transgenic mou...
View Answer

Mating mice from different founder lineages that carry identical transgenes will result in

Correct Answer
verified

Describe the method for creating a knockout mouse by gene targeting. Explain the importance of selectable marker genes in this procedure. What information can a knockout mouse provide?

Correct Answer
Answered by ExamLex AI
Creating a knockout mouse by gene target...
View Answer

Will a cloned calico cat have the exactly the same pattern of orange and black patches on her coat compare with her calico donor? Explain your answer.

Correct Answer
Answered by ExamLex AI
No, a cloned calico cat will not have ex...
View Answer

When making a mouse by gene targeting

Correct Answer
verified

When cloning an organism by nuclear transfer

Correct Answer
verified

Discuss some of the pros and cons of using genetically modified nonhuman primates in experimental medicine.

Correct Answer
Answered by ExamLex AI
Genetically modified nonhuman primates h...
View Answer

You are trying to make a transgenic mouse but there is very poor survivorship of the embryos after introduction of the foreign gene by pronuclear injection. Describe a method you could use to get around this problem.

Correct Answer
Answered by ExamLex AI
One method to improve survivorship of em...
View Answer

Eduardo Kac the transgenic artist produced which of the following?

Correct Answer
verified

What information did the study of crown gall disease provide that was critical for plant genetic engineering?

Correct Answer
verified

The results of nuclear transplantation experiments in Xenopus laevis by John Gurdon and co-workers established the basic principle that

Correct Answer
verified

Gene knockouts often result in embryonic lethality. What strategy do investigators often use to make it possible to study a gene's role later in development?

Correct Answer
verified

You have created a strain of knockout mice. Describe an experiment to confirm that the mice lack expression of the targeted gene at the level of mRNA expression. Show sample positive results.

Correct Answer
Answered by ExamLex AI
To confirm that the knockout mice lack e...
View Answer

Describe an experiment to determine whether the tomato you just bought from the grocery store is genetically modified. Show sample positive results.

Correct Answer
verified
(e.g. design primers specific ...
View Answer

Less than 1% of all nuclear transfers from adult differentiated cells result in normal-appearing cloned offspring. Give possible explanations for this lack of efficiency.

Correct Answer
Answered by ExamLex AI
There are several possible explanations ...
View Answer

Exam 15: Genetically Modified Organisms: Use in Basic and Applied Research

Which is not a method by which one can introduce foreign DNA into a plant cell?

Correct AnswerverifiedShow Answer

All of the following are true of stem cells except:

Correct AnswerverifiedShow Answer

Which of the following is not a suspected reason for the inefficiency/poor outcomes of reproductive cloning?

Correct AnswerverifiedShow Answer

Describe the method for creating a transgenic mouse by pronuclear injection. What information can a transgenic mouse provide?

Correct AnswerAnswered by ExamLex AIThe method for creating a transgenic mou...View AnswerShow Answer

Mating mice from different founder lineages that carry identical transgenes will result in

Correct AnswerverifiedShow Answer

Describe the method for creating a knockout mouse by gene targeting. Explain the importance of selectable marker genes in this procedure. What information can a knockout mouse provide?

Correct AnswerAnswered by ExamLex AICreating a knockout mouse by gene target...View AnswerShow Answer

Will a cloned calico cat have the exactly the same pattern of orange and black patches on her coat compare with her calico donor? Explain your answer.

Correct AnswerAnswered by ExamLex AINo, a cloned calico cat will not have ex...View AnswerShow Answer

When making a mouse by gene targeting

Correct AnswerverifiedShow Answer

When cloning an organism by nuclear transfer

Correct AnswerverifiedShow Answer

Discuss some of the pros and cons of using genetically modified nonhuman primates in experimental medicine.

Correct AnswerAnswered by ExamLex AIGenetically modified nonhuman primates h...View AnswerShow Answer

You are trying to make a transgenic mouse but there is very poor survivorship of the embryos after introduction of the foreign gene by pronuclear injection. Describe a method you could use to get around this problem.

Correct AnswerAnswered by ExamLex AIOne method to improve survivorship of em...View AnswerShow Answer

Eduardo Kac the transgenic artist produced which of the following?

Correct AnswerverifiedShow Answer

What information did the study of crown gall disease provide that was critical for plant genetic engineering?

Correct AnswerverifiedShow Answer

The results of nuclear transplantation experiments in Xenopus laevis by John Gurdon and co-workers established the basic principle that

Correct AnswerverifiedShow Answer

Gene knockouts often result in embryonic lethality. What strategy do investigators often use to make it possible to study a gene's role later in development?

Correct AnswerverifiedShow Answer

You have created a strain of knockout mice. Describe an experiment to confirm that the mice lack expression of the targeted gene at the level of mRNA expression. Show sample positive results.

Correct AnswerAnswered by ExamLex AITo confirm that the knockout mice lack e...View AnswerShow Answer

Describe an experiment to determine whether the tomato you just bought from the grocery store is genetically modified. Show sample positive results.

Correct Answerverified(e.g. design primers specific ...View AnswerShow Answer

Less than 1% of all nuclear transfers from adult differentiated cells result in normal-appearing cloned offspring. Give possible explanations for this lack of efficiency.

Correct AnswerAnswered by ExamLex AIThere are several possible explanations ...View AnswerShow Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
The method for creating a transgenic mou...
View Answer

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
Creating a knockout mouse by gene target...
View Answer

Correct Answer
Answered by ExamLex AI
No, a cloned calico cat will not have ex...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
Genetically modified nonhuman primates h...
View Answer

Correct Answer
Answered by ExamLex AI
One method to improve survivorship of em...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
Answered by ExamLex AI
To confirm that the knockout mice lack e...
View Answer

Correct Answer
verified
(e.g. design primers specific ...
View Answer

Correct Answer
Answered by ExamLex AI
There are several possible explanations ...
View Answer