Question 1

A researcher isolates mRNA from healthy and diseased liver biopsies.  Each mRNA sample is converted into cDNA and subjected to next-generation sequencing.Select all statements that describe this experiment and the possible results.

Accepted Answer

A)   The researcher can identify transcripts that are more abundant in healthy liver than in diseased liver, which are candidate genes for further study.
B)   The researcher can identify transcripts that are less abundant in healthy liver than in diseased liver, which are candidate genes for further study.
C)   This is an RNA-Seq experiment.
D)   This is a DNA microarray analysis.
E)   Some genes' transcripts may be present in both samples but have different splice forms in healthy and diseased liver.

Question 2

________ uses the unique banding patterns of a chromosome to determine the general location of a gene.

Accepted Answer

A)   Physical mapping
B)   Cytogenetic mapping
C)   Linkage mapping
D)   Shotgun sequencing B

Question 3

A microsatellite marker may be used to predict the likelihood that an individual carries a trait for a specific disease.

Accepted Answer

A) True 
 B)False

Question 4

In sequencing by synthesis (SBS)methods,the sequence of a sample is directly determined as nucleotides are incorporated into a DNA strand by DNA polymerase.

Accepted Answer

A) True 
 B)False

Question 5

________ make it possible for researchers to study how an entire genome responds to an environmental stimuli.

Accepted Answer

A)   Gene knockouts
B)   PCR reactions
C)   DNA microarrays
D)   Shotgun sequences

Question 6

Gene chips are being used for which of the following purposes?

Accepted Answer

A)   to examine patterns of gene expression under various environmental conditions
B)   to establish levels of genetic variation
C)   to determine gene expression specific to certain cell types
D)   to identify species of bacteria
E)   All of these choices are possible.

Question 7

RFLP stands for restriction fragment linked position.

Accepted Answer

A) True 
 B)False

Question 8

One map unit of distance always corresponds to 10⁶ base pairs.

Accepted Answer

A) True 
 B)False

Question 9

Part of the Human Genome Project involved sequencing the genomes of model organisms,including bacteria and a plant.

Accepted Answer

A) True 
 B)False

Question 10

In a FISH procedure,the probe being used is labeled with a fluorescent tag.

Accepted Answer

A) True 
 B)False

Question 11

cDNA uses chromosomal DNA as its template.

Accepted Answer

A) True 
 B)False

Question 12

The most common microsatellite in humans is ________.

Accepted Answer

A)   (AT) ₁₀₀
B)   (C
A)  n
C)   (TG) n
D)   (CAT) n
E)   AUG

Question 13

To identify novel DNA binding sites for a protein,it would be best to use ________.

Accepted Answer

A)   pyrosequencing
B)   microsatellites
C)   chromatin immunoprecipitation
D)   ChIP-chip

Question 14

The human genome consists of approximately ________ base pairs of DNA.

Accepted Answer

A)   100,000
B)   1 million
C)   1 billion
D)   3 billion
E)   2 trillion

Question 15

After a metagenomics researcher collects a mixed sample of DNA,what next step allows this DNA to be studied?

Accepted Answer

A)   The DNA fragments are each ligated into a cloning vector to create a DNA library.
B)   The pieces of DNA are ligated together into one complete "genome" so they can be sequenced.
C)   The species responsible for each DNA are identified and individually cultured.
D)   DNA is reconstructed into chromosomes by using the human genome as a scaffold. A

Question 16

A site in the genome that varies between members of the population is said to be ________.

Accepted Answer

A)   monomorphic
B)   polymorphic
C)   trimorphic
D)   neomorphic

Question 17

Match between columns
                        Premises: A site where a single nucleotide is variable between individuals
Individual variation in the size of PCR-amplified fragments
A site containing short repeated sequences
Individual variation in the distance between restriction sites
A marker that is found at only one site within the genome

Accepted Answer

The Answer of Microsatellite and STS and RFLP and SNP is...

Question 18

Pyrosequencing is an example of a high-throughput,next-generation sequencing technology.What is a crucial detail of the procedure?

Accepted Answer

A)   including the enzyme luciferase
B)   mixing the solution of four nucleotides together in even amounts
C)   removing nucleotides from the target DNA one at a time
D)   using large wells to accommodate multiple beads

Question 19

What does physical mapping provide that the other types of genetic mapping do not?

Accepted Answer

A)   recombination distances between genes
B)   number of nucleotide base pairs between genes
C)   relationship of the gene to specific chromosomal banding patterns
D)   frequencies of alleles in a population B

Question 20

An application of FISH technique,where multiple DNA probes are labeled with different fluorescent molecules that bind to a chromosome,is called ________.Using this technique,researchers can determine the sites of the DNA segments along the chromosome.

Accepted Answer

A)   chromosome painting
B)   Giemsa staining
C)   pyrosequencing
D)   physical mapping

Exam 21: Genomics

A researcher isolates mRNA from healthy and diseased liver biopsies. Each mRNA sample is converted into cDNA and subjected to next-generation sequencing.Select all statements that describe this experiment and the possible results.

Correct Answer
verified

________ uses the unique banding patterns of a chromosome to determine the general location of a gene.

Correct Answer
verified
B

A microsatellite marker may be used to predict the likelihood that an individual carries a trait for a specific disease.

Correct Answer
verified

In sequencing by synthesis (SBS)methods,the sequence of a sample is directly determined as nucleotides are incorporated into a DNA strand by DNA polymerase.

Correct Answer
verified

________ make it possible for researchers to study how an entire genome responds to an environmental stimuli.

Correct Answer
verified

Gene chips are being used for which of the following purposes?

Correct Answer
verified

RFLP stands for restriction fragment linked position.

Correct Answer
verified

One map unit of distance always corresponds to 10⁶ base pairs.

Correct Answer
verified

Part of the Human Genome Project involved sequencing the genomes of model organisms,including bacteria and a plant.

Correct Answer
verified

In a FISH procedure,the probe being used is labeled with a fluorescent tag.

Correct Answer
verified

cDNA uses chromosomal DNA as its template.

Correct Answer
verified

The most common microsatellite in humans is ________.

Correct Answer
verified

To identify novel DNA binding sites for a protein,it would be best to use ________.

Correct Answer
verified

The human genome consists of approximately ________ base pairs of DNA.

Correct Answer
verified

After a metagenomics researcher collects a mixed sample of DNA,what next step allows this DNA to be studied?

Correct Answer
verified
A

A site in the genome that varies between members of the population is said to be ________.

Correct Answer
verified

Match each molecular marker with its correct description. Each answer is used only once.

Pyrosequencing is an example of a high-throughput,next-generation sequencing technology.What is a crucial detail of the procedure?

Correct Answer
verified

What does physical mapping provide that the other types of genetic mapping do not?

Correct Answer
verified
B

An application of FISH technique,where multiple DNA probes are labeled with different fluorescent molecules that bind to a chromosome,is called ________.Using this technique,researchers can determine the sites of the DNA segments along the chromosome.

Correct Answer
verified

Exam 21: Genomics

A researcher isolates mRNA from healthy and diseased liver biopsies. Each mRNA sample is converted into cDNA and subjected to next-generation sequencing.Select all statements that describe this experiment and the possible results.

Correct AnswerverifiedShow Answer

________ uses the unique banding patterns of a chromosome to determine the general location of a gene.

Correct AnswerverifiedB

A microsatellite marker may be used to predict the likelihood that an individual carries a trait for a specific disease.

Correct AnswerverifiedShow Answer

In sequencing by synthesis (SBS)methods,the sequence of a sample is directly determined as nucleotides are incorporated into a DNA strand by DNA polymerase.

Correct AnswerverifiedShow Answer

________ make it possible for researchers to study how an entire genome responds to an environmental stimuli.

Correct AnswerverifiedShow Answer

Gene chips are being used for which of the following purposes?

Correct AnswerverifiedShow Answer

RFLP stands for restriction fragment linked position.

Correct AnswerverifiedShow Answer

One map unit of distance always corresponds to 10⁶ base pairs.

Correct AnswerverifiedShow Answer

Part of the Human Genome Project involved sequencing the genomes of model organisms,including bacteria and a plant.

Correct AnswerverifiedShow Answer

In a FISH procedure,the probe being used is labeled with a fluorescent tag.

Correct AnswerverifiedShow Answer

cDNA uses chromosomal DNA as its template.

Correct AnswerverifiedShow Answer

The most common microsatellite in humans is ________.

Correct AnswerverifiedShow Answer

To identify novel DNA binding sites for a protein,it would be best to use ________.

Correct AnswerverifiedShow Answer

The human genome consists of approximately ________ base pairs of DNA.

Correct AnswerverifiedShow Answer

After a metagenomics researcher collects a mixed sample of DNA,what next step allows this DNA to be studied?

Correct AnswerverifiedA

A site in the genome that varies between members of the population is said to be ________.

Correct AnswerverifiedShow Answer

Match each molecular marker with its correct description. Each answer is used only once.

Pyrosequencing is an example of a high-throughput,next-generation sequencing technology.What is a crucial detail of the procedure?

Correct AnswerverifiedShow Answer

What does physical mapping provide that the other types of genetic mapping do not?

Correct AnswerverifiedB

An application of FISH technique,where multiple DNA probes are labeled with different fluorescent molecules that bind to a chromosome,is called ________.Using this technique,researchers can determine the sites of the DNA segments along the chromosome.

Correct AnswerverifiedShow Answer

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B

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A

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B

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