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Exam 10: Recombinant DNA Technology

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Question 21

Multiple Choice

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In yeast,expression of the GAL genes is induced when there is ________ in the culture medium.

A) glucose
B) lactose
C) galactose
D) maltose
E) Any of these

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Question 22

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Expressed sequence tags,or ESTs,

A) are genetic markers generated from cDNA.
B) pertain to expressed genes only.
C) are oligonucleotides derived from mRNA.
D) A and C only
E) All of these

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Question 23

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Briefly explain how Ti plasmids induce crown gall (tumor)growth in plants parasitized by Agrobacterium tumefaciens,highlighting the specific properties that make this plasmid so useful in biotechnology.

DNA sequencing using the dideoxy sequencing method involves PCR reactions using Taq polymerase,which we learned in this chapter can introduce errors because it lacks a proofreading mechanism.What precaution(s)can be taken in genomic or other sequencing projects to minimize the possibility of producing DNA sequences with errors?

DNA fingerprinting can be used to convict criminals,but has had little application in exonerating individuals already convicted.

RT-PCR is a PCR technique used in amplifying DNA copies of mRNA.

________ were the first class of DNA sequence polymorphisms discovered in the human genome.

In a sequenced genome,candidate protein-encoding genes are identified by searching for

When yeast cells are cultured in the presence of both glucose and galactose,the GAL genes are

Somatic cell gene therapy can result in a cure for a genetic disease in an individual as well as any progeny produced by the individual.

________ blots are used to study DNA fragments,while ________ blots are used to study RNA fragments.

How has the Bt protein been used to increase insect-resistance in plants?

Which of the following sequences is considered an STR?

DNA markers are DNA polymorphisms that are useful for genetic mapping.

Please select the best match for each term.

Which of the following techniques is best suited to study protein interactions?

The Guthrie test is used to screen all newborns in the United States for cystic fibrosis.

How does an RFLP help identify sickle-cell anemia?

How does a transcribable vector differ from an expression vector?

Study of P elements in Drosophila has shown that gene regulation can be accomplished by differential mRNA transcript processing.Explain.