Question 1

The reason that many plasmids used in laboratories for transformation experiments contain an ampicillin resistance gene (ampR) is

Accepted Answer

A)  so that the plasmid can replicate in bacterial cells.
B)  so that the plasmid can recombine with the bacterial genome.
C)  to enable the recipient cell to conjugate.
D)  to provide a selectable marker.
E)  to map point mutations.

Question 2

Conjugation by which of the following with an F- cell results in a cell that remains F-?

Accepted Answer

A)  F+ 
B)  Hfr
C)  F
D)  A and C
E)  B and C

Question 3

"Hot spots" are locations in a gene where mutations are found at a relatively high frequency.

Accepted Answer

A) True 
 B)False

Question 4

Which of the following methods can be used to create competent bacterial cells?

Accepted Answer

A)  Treating the cells chemically
B)  Exposing cells to a strong electric field
C)  Making the cell membrane more permeable to DNA
D)  Allowing the culture to enter stationary phase growth
E)  A,B,and C only

Question 5

How did Seymour Benzer use deletion mapping to construct fine-scale maps of the rII region of the T4 bacteriophage genome?

Accepted Answer

He used deletion mutants,which,unlike po...

Question 6

A plasmid such as an F factor that is capable of integrating into the bacterial chromosome is a(n)

Accepted Answer

A)  prophage.
B)  episome.
C)  auxisome.
D)  perisome.
E)  elaiosome.

Question 7

In F+ × F- crosses,none of the bacterial chromosome is transferred; only the F factor is.

Accepted Answer

A) True 
 B)False

Question 8

Which of the following has episomal DNA inserted in the cell's chromosome?

Accepted Answer

A)  An F+ cell
B)  An F' plasmid
C)  An Hfr cell
D)  An F- cell
E)  All except D

Question 9

A merodiploid cell has two copies of one or a few genes and only one copy of all the others.

Accepted Answer

A) True 
 B)False

Question 10

Which of the following statements is true regarding a temperate phage?

Accepted Answer

A)  It can reproduce at warm or cool temperatures.
B)  It is required for transformation.
C)  It can be lytic or lysogenic.
D)  It causes bacterial cells to conjugate.
E)  It reproduces by binary fission.

Question 11

During a cis-trans complementation assay,two lac- strains of bacteria are crossed.The progeny cells exhibit a lac- phenotype.What does this indicate?

Accepted Answer

The two mutations are in diffe...

Question 12

The E.coli chromosome is

Accepted Answer

A)  linear.
B)  circular.
C)  single-stranded.
D)  less than a megabase in length.
E)  compartmentalized within an intracellular membrane.

Question 13

In the case of genetic exchange in bacteria by transformation,there is an exchange of DNA back and forth between cells,and a complete diploid cell is formed.

Accepted Answer

A) True 
 B)False

Question 14

What are temperate bacteriophages?

Accepted Answer

Phages that can alte...

Question 15

In E.coli,λ DNA integrates into which site on the bacterial chromosome?

Accepted Answer

A)  ori
B)  trp
C)  bio
D)  gal
E)  att

Question 16

An arg- strain of E.coli is transformed with a plasmid carrying the wild-type (arg+) gene.The transformed cells are replica-plated to two minimal medium plates: one supplemented with arginine and one lacking arginine.The transformed cells would grow on

Accepted Answer

A)  only the plate supplemented with arginine.
B)  only the plate lacking arginine.
C)  both plates.
D)  neither plate.
E)  the plate supplemented with arginine,but only if a mutation occurred.

Question 17

________ bacterial strains require nutritional supplements to grow in a minimal medium.

Accepted Answer

A)  Autotrophic
B)  Prototrophic
C)  Phototrophic
D)  Heterotrophic
E)  Auxotrophic

Question 18

Cotransductant bacteria can occur if

Accepted Answer

A)  a bacterial cell picks up two fragments of DNA from the environment,each with a different gene.
B)  double crossover occurs between prophage DNA and the bacterial chromosome.
C)  two genes are closely linked enough so that they can be packaged into a phage head and injected into a cell by a single phage.
D)  two genes are introduced into the same bacterium by simultaneous infection with two different phages.
E)  Both C and D

Question 19

E.coli strains that are Hfr

Accepted Answer

A)  are easily mutated.
B)  are susceptible to infection by bacteriophage.
C)  contain the F factor integrated in the bacterial chromosome.
D)  cannot be made competent.
E)  have a low frequency of recombination.

Question 20

Cotransductants can be detected when the transduced genes are

Accepted Answer

A)  closely linked.
B)  far apart on the same chromosome.
C)  on different chromosomes.
D)  mutant.
E)  Both B and D

Exam 15: Genetics of Bacteria and Bacteriophages

"Hot spots" are locations in a gene where mutations are found at a relatively high frequency.

Correct AnswerverifiedShow Answer

E.coli strains that are Hfr

Correct AnswerverifiedShow Answer

In the case of genetic exchange in bacteria by transformation,there is an exchange of DNA back and forth between cells,and a complete diploid cell is formed.

Correct AnswerverifiedShow Answer

An arg- strain of E.coli is transformed with a plasmid carrying the wild-type (arg+) gene.The transformed cells are replica-plated to two minimal medium plates: one supplemented with arginine and one lacking arginine.The transformed cells would grow on

Correct AnswerverifiedShow Answer

A merodiploid cell has two copies of one or a few genes and only one copy of all the others.

Correct AnswerverifiedShow Answer

The reason that many plasmids used in laboratories for transformation experiments contain an ampicillin resistance gene (ampR) is

Correct AnswerverifiedShow Answer

Cotransductants can be detected when the transduced genes are

Correct AnswerverifiedShow Answer

Which of the following has episomal DNA inserted in the cell's chromosome?

Correct AnswerverifiedShow Answer

During a cis-trans complementation assay,two lac- strains of bacteria are crossed.The progeny cells exhibit a lac- phenotype.What does this indicate?

Correct AnswerverifiedThe two mutations are in diffe...View AnswerShow Answer

Which of the following methods can be used to create competent bacterial cells?

Correct AnswerverifiedShow Answer

Conjugation by which of the following with an F- cell results in a cell that remains F-?

Correct AnswerverifiedShow Answer

The E.coli chromosome is

Correct AnswerverifiedShow Answer

What are temperate bacteriophages?

Correct AnswerverifiedPhages that can alte...View AnswerShow Answer

A plasmid such as an F factor that is capable of integrating into the bacterial chromosome is a(n)

Correct AnswerverifiedShow Answer

Which of the following statements is true regarding a temperate phage?

Correct AnswerverifiedShow Answer

How did Seymour Benzer use deletion mapping to construct fine-scale maps of the rII region of the T4 bacteriophage genome?

Correct AnswerverifiedHe used deletion mutants,which,unlike po...View AnswerShow Answer

Cotransductant bacteria can occur if

Correct AnswerverifiedShow Answer

In F+ × F- crosses,none of the bacterial chromosome is transferred; only the F factor is.

Correct AnswerverifiedShow Answer

________ bacterial strains require nutritional supplements to grow in a minimal medium.

Correct AnswerverifiedShow Answer

In E.coli,λ DNA integrates into which site on the bacterial chromosome?

Correct AnswerverifiedShow Answer

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An arg^- strain of E.coli is transformed with a plasmid carrying the wild-type (arg⁺) gene.The transformed cells are replica-plated to two minimal medium plates: one supplemented with arginine and one lacking arginine.The transformed cells would grow on

Correct Answer
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Correct Answer
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The reason that many plasmids used in laboratories for transformation experiments contain an ampicillin resistance gene (amp_R) is

Correct Answer
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Correct Answer
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During a cis-trans complementation assay,two lac^- strains of bacteria are crossed.The progeny cells exhibit a lac^- phenotype.What does this indicate?

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The two mutations are in diffe...
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Conjugation by which of the following with an F^- cell results in a cell that remains F^-?

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Correct Answer
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Phages that can alte...
View Answer

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Correct Answer
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Correct Answer
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He used deletion mutants,which,unlike po...
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In F⁺ × F^- crosses,none of the bacterial chromosome is transferred; only the F factor is.

Correct Answer
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Correct Answer
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Correct Answer
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