Question 1

In Sanger dideoxy DNA sequencing,DNA polymerase I is used to add nucleotides to the ___ end of the growing polynucleotide chain.

Accepted Answer

A)  sticky
B)  blunt
C)  3'
D)  5'
E)  dideoxy nucleotide containing

Question 2

What is the name of the molecule shown in the diagram?

Accepted Answer

A)  adenosine
B)  cytosine
C)  cytidine
D)  cytidine monophosphate
E)  uridine

Question 3

DNA sequencing by the chain-termination method utilizes DNA polymerase I to make a complementary copy of the target or template DNA molecule.A reaction with a 20 bp template and dideoxyadenosine nucleotides as terminators results in the production of a 5 bp fragment.Based on this result,we can conclude that the template contains

Accepted Answer

A)  a cytosine at position 5.
B)  a thymine at position 5.
C)  a cytosine at position 16.
D)  a thymine at position 16.
E)  a uracil at position 5. D

Question 4

Which of the following DNA sequences is considered palindromic?

Accepted Answer

A)  AAGCTT
B)  GAACTT
C)  GAACAA
D)  AAGCAA
E)  AAGTTC

Question 5

In order to perform PCR,which of the following describes the reagents that must be included in the reaction mixture?

Accepted Answer

A)  DNA fragment,primers flanking the region of interest,dNTPs,DNA polymerase
B)  DNA fragment,primers flanking the region of interest,dNTPs,ddNTPS,DNA polymerase
C)  DNA fragment,one primer,dNTPs,DNA polymerase,DNA ligase
D)  DNA fragment,primers flanking the region of interest,dNTPs,DNA Ligase
E)  none of the above

Question 6

DNA sequencing using the Sanger method requires

Accepted Answer

A)  template,primer,DNA polymerase,mRNA,dNTPs,ddNTPs.
B)  template,primer,DNA polymerase,dNTPs,ddNTPs.
C)  template,primer,DNA polymerase,rRNA,dNTPs,ddNTPs.
D)  template,primer,DNA polymerase,mRNA,dNTPs.
E)  none of the above

Question 7

The molecule shown in the diagram is a

Accepted Answer

A)  purine.
B)  dinucleotide.
C)  nucleoside.
D)  nucleotide.
E)  none of the above

Question 8

Matching

-After hybridization,the fragment of interest can be detected by ______.

Accepted Answer

A)   diploid
B)   phosphodiester
C)   probe
D)   pyrimidine
E)   intronsF)  cloningG)  transformedH)  exonsI)  ampicillinJ)  autoradiographyK)  chain-terminatorL)  purineM)  ester J

Question 9

Which of the restriction enzymes listed in the table below produces blunt-end fragments?

Accepted Answer

A)  AluI
B)  BamHI
C)  BglII
D)  EcoRI
E)  HindIII

Question 10

Matching

-The method that Sanger developed for DNA sequencing using dideoxy nucleotides is called the ______ method.

Accepted Answer

A)   diploid
B)   phosphodiester
C)   probe
D)   pyrimidine
E)   intronsF)  cloningG)  transformedH)  exonsI)  ampicillinJ)  autoradiographyK)  chain-terminatorL)  purineM)  ester

Question 11

Matching

-Organisms composed of cells with two sets of chromosomes are called ______ organisms.

Accepted Answer

A)   diploid
B)   phosphodiester
C)   probe
D)   pyrimidine
E)   intronsF)  cloningG)  transformedH)  exonsI)  ampicillinJ)  autoradiographyK)  chain-terminatorL)  purineM)  ester

Question 12

Matching

-A small fragment of labeled DNA or RNA used in a hybridization experiment is called a ______.

Accepted Answer

A)   diploid
B)   phosphodiester
C)   probe
D)   pyrimidine
E)   intronsF)  cloningG)  transformedH)  exonsI)  ampicillinJ)  autoradiographyK)  chain-terminatorL)  purineM)  ester

Question 13

Small circular DNA molecules used to carry foreign DNA fragments are called ________.

Accepted Answer

A)  mRNAs
B)  bacteriophage
C)  clones
D)  plasmids
E)  nucleotides

Question 14

In living organisms,genetic information is most often stored in the form of

Accepted Answer

A)  ribonucleic acid.
B)  deoxyribonucleic acid.
C)  proteins.
D)  enzymes.
E)  deoxynucleotides. B

Question 15

DNA polymerase from Thermus aquaticus is used in PCR because

Accepted Answer

A)  it is a soluble protein.
B)  the genes from Thermus aquaticus are readily distinguished from those of 'normal' organisms.
C)  the enzyme is readily deactivated by heat,effectively halting the reaction.
D)  it is stable at high temperatures.
E)  it is not infectious.

Question 16

In 1944 Avery,MacLeod,and McCarty did experiments with Diplococcus pneumoniae that proved that

Accepted Answer

A)  DNA is double stranded.
B)  DNA contains deoxyribose.
C)  DNA can be transcribed into RNA.
D)  DNA can permanently transform a non-pathogenic strain into a pathogenic strain.
E)  The structure of DNA provides a mechanism for reliable replication.

Question 17

This is the structure of adenine. 
a.Is adenine a purine or a pyrimidine?
b.Which base does adenine base-pair (H-bond)with in DNA?
c.What is the name of the molecule that is composed of adenine linked to the C1' of ribose?
d.Indicate on the drawing through which atom adenine is connected to ribose or deoxyribose.
e.Indicate on the drawing which groups on adenine are involved in base-pairing or H-bonding with its complementary base.

Accepted Answer

a.adenine,guanine,an...

Question 18

Nucleotides in a DNA molecule are linked together through

Accepted Answer

A)  glycosidic bonds.
B)  amide linkages.
C)  hydrogen bonds.
D)  phosphoanhydride bonds.
E)  phosphodiester bonds.

Question 19

Which of the following molecules does not contain an energy rich phosphoanhydride bond?

Accepted Answer

A)  ADP
B)  GDP
C)  AMP
D)  CDP
E)  all of the above

Question 20

Which of the enzymes listed in the table below produce identical sticky ends?

Accepted Answer

A)  AluI and HindIII
B)  AluI and BamHI
C)  HindIII and EcoRI
D)  HindIII and BglII
E)  BamHI and BglII

Exam 3: Nucleotides, nucleic Acids, and Genetic Information

In Sanger dideoxy DNA sequencing,DNA polymerase I is used to add nucleotides to the ___ end of the growing polynucleotide chain.

Correct Answer
verified

What is the name of the molecule shown in the diagram?

Correct Answer
verified

Correct Answer
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D

Which of the following DNA sequences is considered palindromic?

Correct Answer
verified

In order to perform PCR,which of the following describes the reagents that must be included in the reaction mixture?

Correct Answer
verified

DNA sequencing using the Sanger method requires

Correct Answer
verified

The molecule shown in the diagram is a

Correct Answer
verified

Matching -After hybridization,the fragment of interest can be detected by ______.

Correct Answer
verified
J

Which of the restriction enzymes listed in the table below produces blunt-end fragments?

Correct Answer
verified

Matching -The method that Sanger developed for DNA sequencing using dideoxy nucleotides is called the ______ method.

Correct Answer
verified

Matching -Organisms composed of cells with two sets of chromosomes are called ______ organisms.

Correct Answer
verified

Matching -A small fragment of labeled DNA or RNA used in a hybridization experiment is called a ______.

Correct Answer
verified

Small circular DNA molecules used to carry foreign DNA fragments are called ________.

Correct Answer
verified

In living organisms,genetic information is most often stored in the form of

Correct Answer
verified
B

DNA polymerase from Thermus aquaticus is used in PCR because

Correct Answer
verified

In 1944 Avery,MacLeod,and McCarty did experiments with Diplococcus pneumoniae that proved that

Correct Answer
verified

Correct Answer
verified
a.adenine,guanine,an...
View Answer

Nucleotides in a DNA molecule are linked together through

Correct Answer
verified

Which of the following molecules does not contain an energy rich phosphoanhydride bond?

Correct Answer
verified

Which of the enzymes listed in the table below produce identical sticky ends?

Correct Answer
verified

Exam 3: Nucleotides, nucleic Acids, and Genetic Information

In Sanger dideoxy DNA sequencing,DNA polymerase I is used to add nucleotides to the ___ end of the growing polynucleotide chain.

Correct AnswerverifiedShow Answer

What is the name of the molecule shown in the diagram?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedD

Which of the following DNA sequences is considered palindromic?

Correct AnswerverifiedShow Answer

In order to perform PCR,which of the following describes the reagents that must be included in the reaction mixture?

Correct AnswerverifiedShow Answer

DNA sequencing using the Sanger method requires

Correct AnswerverifiedShow Answer

The molecule shown in the diagram is a

Correct AnswerverifiedShow Answer

Matching -After hybridization,the fragment of interest can be detected by ______.

Correct AnswerverifiedJ

Which of the restriction enzymes listed in the table below produces blunt-end fragments?

Correct AnswerverifiedShow Answer

Matching -The method that Sanger developed for DNA sequencing using dideoxy nucleotides is called the ______ method.

Correct AnswerverifiedShow Answer

Matching -Organisms composed of cells with two sets of chromosomes are called ______ organisms.

Correct AnswerverifiedShow Answer

Matching -A small fragment of labeled DNA or RNA used in a hybridization experiment is called a ______.

Correct AnswerverifiedShow Answer

Small circular DNA molecules used to carry foreign DNA fragments are called ________.

Correct AnswerverifiedShow Answer

In living organisms,genetic information is most often stored in the form of

Correct AnswerverifiedB

DNA polymerase from Thermus aquaticus is used in PCR because

Correct AnswerverifiedShow Answer

In 1944 Avery,MacLeod,and McCarty did experiments with Diplococcus pneumoniae that proved that

Correct AnswerverifiedShow Answer

Correct Answerverifieda.adenine,guanine,an...View AnswerShow Answer

Nucleotides in a DNA molecule are linked together through

Correct AnswerverifiedShow Answer

Which of the following molecules does not contain an energy rich phosphoanhydride bond?

Correct AnswerverifiedShow Answer

Which of the enzymes listed in the table below produce identical sticky ends?

Correct AnswerverifiedShow Answer

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D

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J

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B

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a.adenine,guanine,an...
View Answer

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