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What are the final products of aerobic catabolism?


A) pyruvate and H2O.
B) acetyl-CoA and CO2.
C) CO2 and H2O.
D) pyruvate and acetyl-CoA.
E) ammonia and O2.

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What is the relationship between photoautotrophs and heterotrophic cells?


A) Photoautotrophs use light energy for synthesis of organic molecules while heterotrophs metabolize organic substances for fuel and building blocks.
B) photoautotrophs use organic compounds as a source of carbon while heterotrophs use CO2 as a source of carbon.
C) Photoautotrophs synthesize CO2 while heterotrophs synthesize O2.
D) Heterotrophs convert solar energy into chemical energy while photoautotrophs break down organic molecules.
E) There is no relationship between the two.

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A

What vitamin is needed for coagulation of blood and what must happen for it to be functional?


A) vitamin D, a co-translational modification of serine.
B) vitamin K, a posttranslational carboxylation of glutamate.
C) vitamin K, a co-translational carboxylation of aspartate.
D) vitamin D, a posttranslational glycosylation of serine.
E) vitamin E, a posttranslational glycosylation of tyrosine.

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Dehydrogenases are enzymes that:


A) move hydrogens within the molecule.
B) add hydrogens across double bonds.
C) transfer hydrogens between substrates.
D) transfer hydride ions to NAD+ (or NADP+) and release a proton.
E) all are true.

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Pyridoxal-5-phosphate (PLP) is involved in all EXCEPT:


A) aminotransferases (transaminases) .
B) α- and β-decarboxylations.
C) β- and γ-eliminations.
D) racemizations.
E) all of the above.

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Discuss an experiment that used heavy isotopes to elucidate a metabolic pathway.​

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18O was used as a tracer of the fate of the oxygen atoms in water and carbon dioxide to determine whether the atmospheric oxygen produced in photosynthesis arose from H2O, CO2, or both: ​ 11eaa634_fd88_c2ea_842f_152d0741b43b_TB3787_00 ​ If 18O-labeled CO2 was presented to a green plant carrying out photosynthesis, none of the 18O was found in O2. Curiously, it was recovered as H218O. In contrast, when plants fixing CO2 were equilibrated with H218O, 18O2 was evolved. These latter labeling experiments established that photosynthesis is best described by the equation ​ 11eaa634_fd88_e9fb_842f_13275ed39e6c_TB3787_00 ​ That is, in the process of photosynthesis, the two oxygen atoms in O2 come from two H2O molecules. One O is lost from CO2 and appears in H2O, and the other O of CO2 is retained in the carbohydrate product. Two of the four H atoms are accounted for in (CH2O), and two reduce the O lost from CO2 to H2O. ​

The dehydrogenase enzymes facilitate the direct transfer of hydride anion. Which of the following coenzymes is required for this transfer?


A) biotin
B) folic acid
C) lipoic acid
D) TPP
E) NAD+

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All are characteristics of metabolism EXCEPT:


A) a process which synthesizes either energy or complex cellular substances.
B) a process of intermediates.
C) the conversion of food energy into energy of motion.
D) a free-flow unregulated process.
E) a process responding to the momentary energy requirements of a cell.

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____ functions to couple acyl-group transfer and electron transfer during oxidation and decarboxylations of ____.


A) biotin; propionate
B) lipoate; propionate
C) lipoate; α-keto acids
D) TPP; α-keto acids
E) TPP; acetyl-CoA

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All are characteristics of anabolism EXCEPT:


A) assembly of complex molecules.
B) formation of new covalent bonds.
C) ATP provides energy.
D) NADPH is an electron donor.
E) all are true.

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For the following reaction, what coenzyme is missing from the reactants? For the following reaction, what coenzyme is missing from the reactants?   A)  FADH<sub>2</sub> B)  NAD<sup>+</sup> C)  acetyl CoA D)  NADH E)  NADPH


A) FADH2
B) NAD+
C) acetyl CoA
D) NADH
E) NADPH

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Thioesters have higher ____ than that of oxygen esters.


A) mobility
B) electronegativity
C) resonance
D) positive ΔG
E) group transfer potential

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The enzymes of glycolysis and those of the citric acid cycle are found in which cellular compartments in muscle cells?


A) both in the cytoplasm
B) glycolysis in cytoplasm and citric acid cycle in mitochondria
C) both in mitochondria
D) glycolysis in cytoplasm and citric acid cycle in lumen of the endoplasmic reticulum
E) both in the lumen of the endoplasmic reticulum

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Energy is generally extracted from phototrophs and chemotrophs by:


A) hydrolysis reactions.
B) condensation-cleavage reactions.
C) oxidative-reduction reactions.
D) acid-base reactions.
E) phosphorylation reactions.

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Likely the most ancient of the metabolic pathways in which energy is released from glucose and captured in the form of ATP under anaerobic conditions is called:


A) photooxidation.
B) oxidative phosphorylation.
C) citric acid cycle.
D) glycolysis.
E) none of the above.

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All of the following are examples of energy transduction EXCEPT:


A) dehydrogenase catalyzed reactions
B) isomerase reactions
C) oxidative phosphorylation
D) NAD+- NADH shuttle
E) all of the above are correct

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Some species can adapt to anaerobic conditions by substituting other electron acceptors for oxygen in their energy-producing pathways and are called:


A) aerobes.
B) obligate aerobes.
C) facultative anaerobes.
D) obligate anaerobes.
E) none of the above.

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The definition of an amphibolic pathway is:


A) a pathway found in animals living in both land and water.
B) a pathway that is carried out both in water and organic solvents.
C) a pathway found on both sides of the mitochondrial membrane.
D) a pathway that is both anabolic and catabolic in nature.
E) all of the above.

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D

Excess carbohydrate and fat in the human diet is stored as triacylglycerols. The risk of which of the following would be increased in an individual who consistently consumes too much fat and carbohydrate?


A) atherosclerosis
B) heart disease
C) colon cancer
D) breast cancer
E) all of the above

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______ is often used in carboxylation reactions while ____ is often used in decarboxylation reactions.


A) thiamine pyrophosphate; lipoate
B) lipoate; biotin
C) biotin; thiamine pyrophosphate
D) pyridoxal-5-phosphate; biotin
E) lipoate; pyridoxal-5-phosphate

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