Question 1

Which of the following enzymes is the FIRST to bind to the DNA sequences at the origin of replication?

Accepted Answer

A)  DNA polymerase
B)  DNA helicase
C)  DNA primase
D)  single-strand binding protein
E)  DNA ligase

Question 2

Which of the following is considered a transgenic organism?

Accepted Answer

A)  a bacterium that has received genes by conjugation
B)  a human given a blood-clotting factor produced in bacterial cells
C)  a fern grown in cell culture from a single fern root cell
D)  a rat with rabbit hemoglobin genes
E)  a human treated with insulin produced by bacteria

Question 3

During PCR, where does the energy come from that adds the nucleotide to the growing DNA strand?

Accepted Answer

A)  Taq polymerase
B)  the primers
C)  the elevated temperature of the PCR reaction
D)  the incoming nucleotides
E)  ATP

Question 4

If you were to use CRISPR to cleave DNA at sites flanking a gene, and in this way produce a deletion of the gene, how many CRISPR RNAs would you need?

Accepted Answer

A)  1
B)  2
C)  3
D)  4

Question 5

DNA renaturation (hybridization)  can be used to:

Accepted Answer

A)  determine the degree of similarity between two DNA samples.
B)  cleave long fragments of DNA into smaller fragments for analysis.
C)  amplify small samples of DNA by replicating them.
D)  determine the nucleotide sequence of a DNA strand.
E)  None of the other answer options is correct.

Question 6

The size of the yeast genome is 1.2 × 10 7 and the GC content is 38%. Approximately how many EcoR1 restriction fragments would you expect to get from cutting the yeast genome with EcoR1?

Accepted Answer

A)  4000
B)  1000
C)  1 × 106
D)  500
E)  40,000

Question 7

An organism is found in which the template RNA sequence in the telomerase is   5'-CCAUAA-3'.
 What is the DNA sequence of the organism's telomeres?

Accepted Answer

A)    3 ’ −… CCAUAACCAUAA …−5 ’ 5′−… GGUAUUGGUAUU …−3 ’ \begin{array}{l}3 	ext { &#x27; }-\ldots 	ext { CCAUAACCAUAA } \ldots-5 	ext { &#x27; } \5^{\prime}-\ldots 	ext { GGUAUUGGUAUU } \ldots-3 	ext { &#x27; }\end{array}3 ’ −… CCAUAACCAUAA …−5 ’ 5′−… GGUAUUGGUAUU …−3 ’ ​ 
B)    3′−… GGUAUUGGUAUU …−5 ’ 5′−… CCAUAACCAUAA ...−3 ’ \begin{array}{l}3 ^{\prime}-\ldots 	ext { GGUAUUGGUAUU } \ldots-5 	ext { &#x27; } \5^{\prime}-\ldots 	ext { CCAUAACCAUAA } . . .-3 	ext { &#x27; }\end{array}3′−… GGUAUUGGUAUU …−5 ’ 5′−… CCAUAACCAUAA ...−3 ’ ​ 
C)    3 ’-... CCAUAACCATAA...-5’ 5′−… GGTATTGGTATT ...-3’ \begin{array}{l}3 	ext { &#x27;-... CCAUAACCATAA...-5&#x27; } \5^{\prime}-\ldots 	ext { GGTATTGGTATT ...-3&#x27; }\end{array}3 ’-... CCAUAACCATAA...-5’ 5′−… GGTATTGGTATT ...-3’ ​ 
D)     3’-...GGTATTGGTATT...-5’ 	ext { 3&#x27;-...GGTATTGGTATT...-5&#x27; } 3’-...GGTATTGGTATT...-5’    5’-... ???????????A...-3’ 	ext { 5&#x27;-... ???????????A...-3&#x27; } 5’-... ???????????A...-3’

Question 8

Given the actions listed below, which are all involved in PCR, select the answer that places them in the CORRECT order. extension
Initiation
Denaturation
Termination
Annealing

Accepted Answer

A)  initiation, extension, termination
B)  denaturation, annealing, extension
C)  denaturation, extension, termination
D)  initiation, denaturation, extension
E)  annealing, initiation, termination

Question 9

_____ is the first enzyme to synthesize a nucleic acid at a replication fork.

Accepted Answer

A)  RNA primase
B)  Helicase
C)  Topoisomerase II
D)  DNA polymerase

Question 10

Given that a chromosome is 60,000,000 bases in length, and DNA polymerase functions at a rate of 50 base pairs per second, what is the minimum number of origins of replication required on that chromosome to allow replication to complete if S phase lasts for 5.5 hours?

Accepted Answer

A)  one
B)  about 30
C)  about 300
D)  about 3000
E)  about 30,000

Question 11

The extrachromosomal DNA often found in bacteria is called a:

Accepted Answer

A)  vector.
B)  plasmid.
C)  telomere.
D)  restriction enzyme.

Question 12

Why is DNA ligase important for a cell?

Accepted Answer

A)  It joins Okazaki fragments.
B)  It relieves the stress of DNA unwinding.
C)  It joins DNA together from different origins of replication.
D)  It unwinds DNA.

Question 13

What is the name of the enzyme that separates the two strands of DNA during replication?

Accepted Answer

A)  DNA polymerase
B)  DNA ligase
C)  primase
D)  helicase
E)  topoisomerase

Question 14

The enzyme responsible for joining Okazaki fragments together during DNA replication is:

Accepted Answer

A)  DNA polymerase.
B)  helicase.
C)  topoisomerase II.
D)  DNA ligase.
E)  DNA replicase.

Question 15

Restriction enzymes are an essential component of PCR.

Accepted Answer

A) True 
 B)False

Question 16

During the process of PCR, what plays the role in the test tube that helicase plays in the cell?

Accepted Answer

A)  dNTPs
B)  heat
C)  polymerase
D)  oligonucleotides

Question 17

To cells that are defective in primer removal, you add fluorescent ribonucleotides when the cells are undergoing DNA replication. In this case, you observe that one strand glows more than the other not only near the replication fork but also at intervals along its length. Which strand glows in this way and why?

Accepted Answer

A)  The leading strand glows in this way because it is synthesized continuously.
B)  The leading strand glows in this way because its RNA primers are widely spaced.
C)  The lagging strand glows in this way because it is synthesized continuously.
D)  The lagging strand glows in this way because its RNA primers are closely spaced.

Question 18

In recombinant DNA experiments, _____ is used to cut pieces of DNA, and _____ joins these segments to form recombinant DNA.

Accepted Answer

A)  a restriction enzyme; DNA ligase
B)  a RNA polymerase; a restriction enzyme
C)  a DNA polymerase; DNA ligase
D)  DNA ligase; a restriction enzyme
E)  DNA ligase; a plasmid

Question 19

Okazaki fragments are ligated into a continuous DNA molecule after replication is completed.

Accepted Answer

A) True 
 B)False

Question 20

Suppose you add fluorescent ribonucleotides to a cell undergoing DNA replication so that the RNA primers used in DNA synthesis glow when viewed with a fluorescent microscope. You notice that, near each replication fork, one strand glows more than the other. Which strand is it that glows more, and why?

Accepted Answer

A)  The leading strand glows more because it is elongated nearest the replication fork.
B)  The leading strand glows more because it forms the "trombone loop."
C)  The lagging strand glows more because its RNA primer is nearer the replication fork.
D)  The lagging strand glows more because it forms the "trombone loop."

Exam 12: Dna Replication and Manipulation

In recombinant DNA experiments, _ is used to cut pieces of DNA, and _ joins these segments to form recombinant DNA.

Correct Answer
verified

During the process of PCR, what plays the role in the test tube that helicase plays in the cell?

Correct Answer
verified

Restriction enzymes are an essential component of PCR.

Correct Answer
verified

Which of the following is considered a transgenic organism?

Correct Answer
verified

Correct Answer
verified

What is the name of the enzyme that separates the two strands of DNA during replication?

Correct Answer
verified

_____ is the first enzyme to synthesize a nucleic acid at a replication fork.

Correct Answer
verified

The extrachromosomal DNA often found in bacteria is called a:

Correct Answer
verified

Okazaki fragments are ligated into a continuous DNA molecule after replication is completed.

Correct Answer
verified

If you were to use CRISPR to cleave DNA at sites flanking a gene, and in this way produce a deletion of the gene, how many CRISPR RNAs would you need?

Correct Answer
verified

Given the actions listed below, which are all involved in PCR, select the answer that places them in the CORRECT order. extension Initiation Denaturation Termination Annealing

Correct Answer
verified

DNA renaturation (hybridization) can be used to:

Correct Answer
verified

Which of the following enzymes is the FIRST to bind to the DNA sequences at the origin of replication?

Correct Answer
verified

Given that a chromosome is 60,000,000 bases in length, and DNA polymerase functions at a rate of 50 base pairs per second, what is the minimum number of origins of replication required on that chromosome to allow replication to complete if S phase lasts for 5.5 hours?

Correct Answer
verified

During PCR, where does the energy come from that adds the nucleotide to the growing DNA strand?

Correct Answer
verified

Correct Answer
verified

Why is DNA ligase important for a cell?

Correct Answer
verified

The enzyme responsible for joining Okazaki fragments together during DNA replication is:

Correct Answer
verified

The size of the yeast genome is 1.2 × 10⁷ and the GC content is 38%. Approximately how many EcoR1 restriction fragments would you expect to get from cutting the yeast genome with EcoR1?

Correct Answer
verified

An organism is found in which the template RNA sequence in the telomerase is 5'-CCAUAA-3'. What is the DNA sequence of the organism's telomeres?

Correct Answer
verified

Exam 12: Dna Replication and Manipulation

In recombinant DNA experiments, _____ is used to cut pieces of DNA, and _____ joins these segments to form recombinant DNA.

Correct AnswerverifiedShow Answer

During the process of PCR, what plays the role in the test tube that helicase plays in the cell?

Correct AnswerverifiedShow Answer

Restriction enzymes are an essential component of PCR.

Correct AnswerverifiedShow Answer

Which of the following is considered a transgenic organism?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

What is the name of the enzyme that separates the two strands of DNA during replication?

Correct AnswerverifiedShow Answer

_____ is the first enzyme to synthesize a nucleic acid at a replication fork.

Correct AnswerverifiedShow Answer

The extrachromosomal DNA often found in bacteria is called a:

Correct AnswerverifiedShow Answer

Okazaki fragments are ligated into a continuous DNA molecule after replication is completed.

Correct AnswerverifiedShow Answer

If you were to use CRISPR to cleave DNA at sites flanking a gene, and in this way produce a deletion of the gene, how many CRISPR RNAs would you need?

Correct AnswerverifiedShow Answer

Given the actions listed below, which are all involved in PCR, select the answer that places them in the CORRECT order. extension Initiation Denaturation Termination Annealing

Correct AnswerverifiedShow Answer

DNA renaturation (hybridization) can be used to:

Correct AnswerverifiedShow Answer

Which of the following enzymes is the FIRST to bind to the DNA sequences at the origin of replication?

Correct AnswerverifiedShow Answer

Given that a chromosome is 60,000,000 bases in length, and DNA polymerase functions at a rate of 50 base pairs per second, what is the minimum number of origins of replication required on that chromosome to allow replication to complete if S phase lasts for 5.5 hours?

Correct AnswerverifiedShow Answer

During PCR, where does the energy come from that adds the nucleotide to the growing DNA strand?

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Why is DNA ligase important for a cell?

Correct AnswerverifiedShow Answer

The enzyme responsible for joining Okazaki fragments together during DNA replication is:

Correct AnswerverifiedShow Answer

The size of the yeast genome is 1.2 × 10 7 and the GC content is 38%. Approximately how many EcoR1 restriction fragments would you expect to get from cutting the yeast genome with EcoR1?

Correct AnswerverifiedShow Answer

An organism is found in which the template RNA sequence in the telomerase is 5'-CCAUAA-3'. What is the DNA sequence of the organism's telomeres?

Correct AnswerverifiedShow Answer

In recombinant DNA experiments, _ is used to cut pieces of DNA, and _ joins these segments to form recombinant DNA.

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
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Correct Answer
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Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified

The size of the yeast genome is 1.2 × 10⁷ and the GC content is 38%. Approximately how many EcoR1 restriction fragments would you expect to get from cutting the yeast genome with EcoR1?

Correct Answer
verified

Correct Answer
verified